The overwintering females emerge from early April and are active until the end of August. The males emerge from the end of July, and fly to the end of September
Parasites Alfken (1909) cites Sphecodes longulus as a cleptoparasite of this species. Amiet et al. (2001) suggest that Nomada bluethgeni might also be a cleptoparasite of L. marginellum. Westrich (2006) states that the bees Nomada sheppardana and Sphecodes geofrellus as cleptoparasites of this species